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picogreen dsdna quantitation reagent  (Yeasen Biotechnology)

 
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    Structured Review

    Yeasen Biotechnology picogreen dsdna quantitation reagent
    The standard curve was generated by plotting the fluorescence intensity against the <t>dsDNA</t> concentration (ng/mL). Due to the wide dynamic range of the assay, both axes are presented with a break to clearly display the low-concentration region (0–50 ng/mL) and the high-concentration region (50–1,000 ng/mL) on a single graph. Data points represent the mean <t>PicoGreen</t> fluorescence from a single independent experiment, performed with at least three technical replicates per dsDNA concentration. The dsDNA concentrations shown are consistent with those listed in . Standard deviations (SD) were calculated for each concentration, and the coefficients of variation were consistently below 5% across all concentrations tested. The linear regression equation is y = 15.9x + 78.831 (R 2 = 1), where y is PicoGreen fluorescence intensity and x is dsDNA concentration in ng/mL. Unknown concentrations can be calculated as x = (y - 78.831)/15.92. The dsDNA detection range is 1–1,000 ng/mL.
    Picogreen Dsdna Quantitation Reagent, supplied by Yeasen Biotechnology, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/picogreen dsdna quantitation reagent/product/Yeasen Biotechnology
    Average 86 stars, based on 1 article reviews
    picogreen dsdna quantitation reagent - by Bioz Stars, 2026-05
    86/100 stars

    Images

    1) Product Images from "A Cell-Based Protocol to Assess Manganese Content and Relative Transport Activity of Manganese Transporters"

    Article Title: A Cell-Based Protocol to Assess Manganese Content and Relative Transport Activity of Manganese Transporters

    Journal: Bio-protocol

    doi: 10.21769/BioProtoc.5680

    The standard curve was generated by plotting the fluorescence intensity against the dsDNA concentration (ng/mL). Due to the wide dynamic range of the assay, both axes are presented with a break to clearly display the low-concentration region (0–50 ng/mL) and the high-concentration region (50–1,000 ng/mL) on a single graph. Data points represent the mean PicoGreen fluorescence from a single independent experiment, performed with at least three technical replicates per dsDNA concentration. The dsDNA concentrations shown are consistent with those listed in . Standard deviations (SD) were calculated for each concentration, and the coefficients of variation were consistently below 5% across all concentrations tested. The linear regression equation is y = 15.9x + 78.831 (R 2 = 1), where y is PicoGreen fluorescence intensity and x is dsDNA concentration in ng/mL. Unknown concentrations can be calculated as x = (y - 78.831)/15.92. The dsDNA detection range is 1–1,000 ng/mL.
    Figure Legend Snippet: The standard curve was generated by plotting the fluorescence intensity against the dsDNA concentration (ng/mL). Due to the wide dynamic range of the assay, both axes are presented with a break to clearly display the low-concentration region (0–50 ng/mL) and the high-concentration region (50–1,000 ng/mL) on a single graph. Data points represent the mean PicoGreen fluorescence from a single independent experiment, performed with at least three technical replicates per dsDNA concentration. The dsDNA concentrations shown are consistent with those listed in . Standard deviations (SD) were calculated for each concentration, and the coefficients of variation were consistently below 5% across all concentrations tested. The linear regression equation is y = 15.9x + 78.831 (R 2 = 1), where y is PicoGreen fluorescence intensity and x is dsDNA concentration in ng/mL. Unknown concentrations can be calculated as x = (y - 78.831)/15.92. The dsDNA detection range is 1–1,000 ng/mL.

    Techniques Used: Generated, Fluorescence, Concentration Assay



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    The standard curve was generated by plotting the fluorescence intensity against the <t>dsDNA</t> concentration (ng/mL). Due to the wide dynamic range of the assay, both axes are presented with a break to clearly display the low-concentration region (0–50 ng/mL) and the high-concentration region (50–1,000 ng/mL) on a single graph. Data points represent the mean <t>PicoGreen</t> fluorescence from a single independent experiment, performed with at least three technical replicates per dsDNA concentration. The dsDNA concentrations shown are consistent with those listed in . Standard deviations (SD) were calculated for each concentration, and the coefficients of variation were consistently below 5% across all concentrations tested. The linear regression equation is y = 15.9x + 78.831 (R 2 = 1), where y is PicoGreen fluorescence intensity and x is dsDNA concentration in ng/mL. Unknown concentrations can be calculated as x = (y - 78.831)/15.92. The dsDNA detection range is 1–1,000 ng/mL.
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    The characterizations of in vitro sonodynamic effects and cGAS-STING pathway activation. 4T1 cells were inoculated and incubated with PBSN38-CUR for 24 h, followed by characterization of (A) cytotoxicity, (B - D) apoptosis, and DNA damage after US irradiation (frequency: 3 MHz, intensity: 1.5 W cm −2 , duty cycle: 50 %, duration time: 1 min). Scale bar: 100 μm. (E) Release behavior of cellular <t>dsDNA</t> after various treatments. (F) Expression of STING, TBK1, and IRF3 proteins and their corresponding phosphorylated proteins after different treatments. ( G ) Maturation characterization of DCs after various treatments. Data were expressed as mean ± standard deviation of biological replicates (n = 3). One-way analysis of variance (ANOVA) followed by the post-Tukey's multiple comparisons test was used to compare multiple groups.
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    Image Search Results


    The standard curve was generated by plotting the fluorescence intensity against the dsDNA concentration (ng/mL). Due to the wide dynamic range of the assay, both axes are presented with a break to clearly display the low-concentration region (0–50 ng/mL) and the high-concentration region (50–1,000 ng/mL) on a single graph. Data points represent the mean PicoGreen fluorescence from a single independent experiment, performed with at least three technical replicates per dsDNA concentration. The dsDNA concentrations shown are consistent with those listed in . Standard deviations (SD) were calculated for each concentration, and the coefficients of variation were consistently below 5% across all concentrations tested. The linear regression equation is y = 15.9x + 78.831 (R 2 = 1), where y is PicoGreen fluorescence intensity and x is dsDNA concentration in ng/mL. Unknown concentrations can be calculated as x = (y - 78.831)/15.92. The dsDNA detection range is 1–1,000 ng/mL.

    Journal: Bio-protocol

    Article Title: A Cell-Based Protocol to Assess Manganese Content and Relative Transport Activity of Manganese Transporters

    doi: 10.21769/BioProtoc.5680

    Figure Lengend Snippet: The standard curve was generated by plotting the fluorescence intensity against the dsDNA concentration (ng/mL). Due to the wide dynamic range of the assay, both axes are presented with a break to clearly display the low-concentration region (0–50 ng/mL) and the high-concentration region (50–1,000 ng/mL) on a single graph. Data points represent the mean PicoGreen fluorescence from a single independent experiment, performed with at least three technical replicates per dsDNA concentration. The dsDNA concentrations shown are consistent with those listed in . Standard deviations (SD) were calculated for each concentration, and the coefficients of variation were consistently below 5% across all concentrations tested. The linear regression equation is y = 15.9x + 78.831 (R 2 = 1), where y is PicoGreen fluorescence intensity and x is dsDNA concentration in ng/mL. Unknown concentrations can be calculated as x = (y - 78.831)/15.92. The dsDNA detection range is 1–1,000 ng/mL.

    Article Snippet: Picogreen dsDNA quantitation reagent (YEASEN, catalog number: 12641ES04) Western blotting 17.

    Techniques: Generated, Fluorescence, Concentration Assay

    The characterizations of in vitro sonodynamic effects and cGAS-STING pathway activation. 4T1 cells were inoculated and incubated with PBSN38-CUR for 24 h, followed by characterization of (A) cytotoxicity, (B - D) apoptosis, and DNA damage after US irradiation (frequency: 3 MHz, intensity: 1.5 W cm −2 , duty cycle: 50 %, duration time: 1 min). Scale bar: 100 μm. (E) Release behavior of cellular dsDNA after various treatments. (F) Expression of STING, TBK1, and IRF3 proteins and their corresponding phosphorylated proteins after different treatments. ( G ) Maturation characterization of DCs after various treatments. Data were expressed as mean ± standard deviation of biological replicates (n = 3). One-way analysis of variance (ANOVA) followed by the post-Tukey's multiple comparisons test was used to compare multiple groups.

    Journal: Materials Today Bio

    Article Title: Ultrasound-triggered carrier-free nanoprodrugs activate cGAS-STING pathway to enhance tumor-targeting chemo-immunotherapy

    doi: 10.1016/j.mtbio.2026.102858

    Figure Lengend Snippet: The characterizations of in vitro sonodynamic effects and cGAS-STING pathway activation. 4T1 cells were inoculated and incubated with PBSN38-CUR for 24 h, followed by characterization of (A) cytotoxicity, (B - D) apoptosis, and DNA damage after US irradiation (frequency: 3 MHz, intensity: 1.5 W cm −2 , duty cycle: 50 %, duration time: 1 min). Scale bar: 100 μm. (E) Release behavior of cellular dsDNA after various treatments. (F) Expression of STING, TBK1, and IRF3 proteins and their corresponding phosphorylated proteins after different treatments. ( G ) Maturation characterization of DCs after various treatments. Data were expressed as mean ± standard deviation of biological replicates (n = 3). One-way analysis of variance (ANOVA) followed by the post-Tukey's multiple comparisons test was used to compare multiple groups.

    Article Snippet: Picogreen dsDNA quantitation reagent was purchased from Yeasen Biotechnology (Shanghai) Co., Ltd. InVivoMAb anti-mouse PD-L1 (B7-H1) antibody was purchased from Bioxcell.

    Techniques: In Vitro, Activation Assay, Incubation, Irradiation, Expressing, Standard Deviation